prikaz prve stranice dokumenta Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems
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Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems
International Journal of Legal Medicine, 133 (2019), 5; 1369-1380. https://doi.org/10.1007/s00414-019-02110-4
Zavala, Elena I.; Rajagopal, Swetha; Perry, George H.; Kruzic, Ivana; Bašić, Željana; Parsons, Thomas J.; Holland, Mitchell M.

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Zavala, E. I., Rajagopal, S., Perry, G. H., Kruzic, I., Bašić, Ž., Parsons, T. J. i Holland, M. M. (2019). Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems. International Journal of Legal Medicine, 133. (5), 1369-1380. doi: 10.1007/s00414-019-02110-4

Zavala, Elena I., et al. "Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems." International Journal of Legal Medicine, vol. 133, br. 5, 2019, str. 1369-1380. https://doi.org/10.1007/s00414-019-02110-4

Zavala, Elena I., Swetha Rajagopal, George H. Perry, Ivana Kruzic, Željana Bašić, Thomas J. Parsons i Mitchell M. Holland. "Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems." International Journal of Legal Medicine 133, br. 5 (2019): 1369-1380. https://doi.org/10.1007/s00414-019-02110-4

Zavala, E. I., et al. (2019) 'Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems', International Journal of Legal Medicine, 133(5), str. 1369-1380. doi: 10.1007/s00414-019-02110-4

Zavala EI, Rajagopal S, Perry GH, Kruzic I, Bašić Ž, Parsons TJ, i sur.. Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems. International Journal of Legal Medicine [Internet]. 2019. [pristupljeno 06.12.2024.];133(5):1369-1380. doi: 10.1007/s00414-019-02110-4

E. I. Zavala, et al., "Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems", International Journal of Legal Medicine, vol. 133, br. 5, str. 1369-1380, 2019. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:227:769452. [Citirano: 06.12.2024.]

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Naslov (engleski)Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems
AutorElena I. Zavala
AutorSwetha Rajagopal
AutorGeorge H. Perry
AutorIvana Kruzic
AutorŽeljana Bašić
AutorThomas J. Parsons
AutorMitchell M. Holland
Autorova ustanovaSveučilište u Splitu
Sveučilišni odjel za forenzične znanosti
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Znanstveno / umjetničko
područje, polje i grana		HUMANISTIČKE ZNANOSTI
Etnologija i antropologija
Sažetak (engleski)
Biological samples, including skeletal remains exposed to environmental insults for extended periods of time, exhibit increasing levels of DNA damage and fragmentation. Human forensic identification methods typically use a combination of mitochondrial (mt) DNA sequencing and short tandem repeat (STR) analysis, which target segments of DNA ranging from 80 to 500 base pairs (bps). Larger templates are often unavailable as skeletal samples age and the associated DNA degrades. Single-nucleotide polymorphism (SNP) loci target shorter templates and may serve as a solution to the problem. Recently developed assays for STR and SNP analysis using a massively parallel sequencing approach, such as the ForenSeq kit (Verogen, San Diego, CA), offer a means for generating results from degraded samples as they target templates down to 60 to 170 bps. We performed a modeling study that demonstrates that SNPs can increase the significance of an identification when analyzing DNA down to an average size of 100 bps for input amounts between 0.375 and 1 ng of nuclear DNA. Observations from this study were then compared with human skeletal material results (n = 14, ninth to eighteenth centuries), which further demonstrated the utility of the ForenSeq kit for degraded samples. The robustness of the Promega PowerSeq™ Mito System was also tested with human skeletal remains (n = 70, ninth to eighteenth centuries), resulting in successful coverage of 99.29% of the mtDNA control region at 50× coverage or more. This was accompanied by modifications to a mainstream DNA extraction technique for skeletal remains that improved recovery of shorter templates.
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Legal Medicine
Brojčani podacivol. 133, br. 5, str. 1369-1380
p-ISSN0937-9827
e-ISSN1437-1596
DOIhttps://doi.org/10.1007/s00414-019-02110-4
URN:NBNurn:nbn:hr:227:769452
Datum objave publikacije2019
URL dokumentahttp://link.springer.com/10.1007/s00414-019-02110-4
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2024-06-10 12:41:10