Objective: The objective of this paper is to determine the frequency of the presence of persistent metopic suture in the adult population of Salona and to contribute to the research of persistent metopic suture and epigenetic characteristics in anthropology and forensics. Methods: Skeletal remains from the Solin-Smiljanovac archaeological site were examined. Of the skeletal remains of 629 persons, 142 of them (68 women, 66 men, 8 of unknown sex) belonged to adults (older than 18) and had a preserved frontal bone on which the presence of a persistent metopic suture could be determined and whether it was a complete or incomplete. The incomplete metopic suture was then further defined by type (lower, middle or upper). The types below are further divided by shape (U, V, H, Y, inverted Y, side-to-side, radial, linear and double). In the research, no distinction was made between the lower metopic suture and the supranasal suture. Data on sex, age, preservation of the frontal bone, and the type and shape of the metopic suture were recorded in tables, and the metopic sutures were photographed. Results: Persistent metopic suture was recorded in 78 skulls (54.93%), in 45 male and 35 female skulls. Incomplete metopic suture/supranasal suture was recorded in 67 skulls, 36 male and 29 female skulls (47.18%). Metopism was recorded on 10 skulls (5 male, 5 female). Conclusion: The presence of a persistent metopic suture in Salona is similar to that recorded in studies conducted on geographically and temporally close populations. Further research on the territory of today's Republic of Croatia would provide insight into the presence of a persistent metopic suture throughout the history of Croatia and would point to the importance of studying epigenetic characteristics.