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master's thesis
Osjetljivost i specifičnost Seratec HemDirect testa na krv životinja koje nisu sisavci
2022. urn:nbn:hr:227:144874
Svetina, Antonija
University of Split
University Department of Forensic Sciences

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Svetina, A. (2022). Osjetljivost i specifičnost Seratec HemDirect testa na krv životinja koje nisu sisavci (Master's thesis). Split: University of Split, University Department of Forensic Sciences. Retrieved from https://urn.nsk.hr/urn:nbn:hr:227:144874

Svetina, Antonija. "Osjetljivost i specifičnost Seratec HemDirect testa na krv životinja koje nisu sisavci." Master's thesis, University of Split, University Department of Forensic Sciences, 2022. https://urn.nsk.hr/urn:nbn:hr:227:144874

Svetina, Antonija. "Osjetljivost i specifičnost Seratec HemDirect testa na krv životinja koje nisu sisavci." Master's thesis, University of Split, University Department of Forensic Sciences, 2022. https://urn.nsk.hr/urn:nbn:hr:227:144874

Svetina, A. (2022). 'Osjetljivost i specifičnost Seratec HemDirect testa na krv životinja koje nisu sisavci', Master's thesis, University of Split, University Department of Forensic Sciences, accessed 14 November 2024, https://urn.nsk.hr/urn:nbn:hr:227:144874

Svetina A. Osjetljivost i specifičnost Seratec HemDirect testa na krv životinja koje nisu sisavci [Master's thesis]. Split: University of Split, University Department of Forensic Sciences; 2022 [cited 2024 November 14] Available at: https://urn.nsk.hr/urn:nbn:hr:227:144874

A. Svetina, "Osjetljivost i specifičnost Seratec HemDirect testa na krv životinja koje nisu sisavci", Master's thesis, University of Split, University Department of Forensic Sciences, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:227:144874

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Metadata
TitleOsjetljivost i specifičnost Seratec HemDirect testa na krv životinja koje nisu sisavci
Title (english)Sensitivity and specificity of Seratec HemDirect Test on nonmammalian blood
AuthorAntonija Svetina
MentorDamir Marjanović (mentor)
MentorJosip Crnjac (komentor)
Committee memberSnježana Štambuk (predsjednik povjerenstva)
Committee memberNevena Aljinović (član povjerenstva)
Committee memberDamir Marjanović (član povjerenstva)
GranterUniversity of Split
University Department of Forensic Sciences
Split
Defense date and country2022-09-22, Croatia
Scientific / art field,
discipline and subdiscipline		INTERDISCIPLINARY AREAS OF KNOWLEDGE
Cognitive Science (Natural, Technical, Biomedical and Healthcare, Social and Humanistic Sciences)
Abstract
Cilj: Cilj ovog rada je bio provesti dodatnu validaciju Seratec HemDirect testa odnosno ispitati osjetljivost i specifičnost testa za krv te utvrditi postoje li životinjske vrste čija krv daje lažno pozitivan rezultat na ovom testu koji bi trebao biti specifičan za krv primata. Metode: Istraživanje je obuhvatilo 10 uzoraka venske krvi životinjskih vrsta. Testom Seratec HemDirect su analizirani svi uzorci krvi. Iz pune krvi dodavanjem dvostruko destilirane vode su pripremljena razrjeđenja (1/5, 1/10, 1/20, 1/40). Nakon toga su uzorci svakog razrjeđenja volumena 50 μL stavljeni na bijelu pamučnu tkaninu te sušeni na sobnoj temperaturi. Zatim je slijedilo rezanje 1/4 uzorka koji se ekstrahirao u puferu 30 minuta. Poslije ekstrakcije potrebno je nakapati tri kapi svakog uzorka u jažicu za ispitivanje te pričekati 10 minuta kada se rezultati očitavaju i bilježe. Rezultati: U analiziranim uzorcima razrijeđene krvi svih životinjskih vrsta, Seratec HemDirect test je pokazao negativne rezultate. Zaključak: Seratec HemDirect test je pouzdan preliminarni test koji pokazuje visoku osjetljivost na ljudsku krv, te na životinjsku ne reagira. Upotreba ovog testa u forenzici može biti od velikog značaja za daljnji tijek istrage.
Abstract (english)
Aim: The aim of this study was to further validate the Seratec HemDirect test, test the sensitivity and specificity of the blood test and determine if there are any animal species whose blood gives false positive results on this test which should be specific to primate blood. Methods: The study included 10 samples of venous blood of animal species. Seratec HemDirect tested all blood samples. Dilutions have been prepared from the whole blood by adding double distilled water (1/5, 1/10, 1/20, 1/40). After that, samples of each 50 μL dilution were placed on a white cotton fabric and dried at room temperature. Then followed by the cutting of 1/4 of the sample, which was extracted in the buffer for 30 minutes. After extraction three drops of each sample should be added into the test well and wait 10 minutes before the results are read and recorded. Results: In the analyzed samples of diluted blood of all animal species, Seratec HemDirect test showed negative results. Conclusion: Seratec HemDirect test is a reliable preliminary test that shows high sensitivity to human blood and does not respond to animal blood. The use of this test in forensics can be of great importance for the further course of the investigation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:227:144874
Study programmeTitle: Forensic Sciences
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni magistar/sveučilišna magistra forenzike (sveučilišni magistar/sveučilišna magistra forenzike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-07-20 07:02:44