prikaz prve stranice dokumenta Primjena direktne STR amplifikacije u forenzičnim znanostima
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master's thesis
Primjena direktne STR amplifikacije u forenzičnim znanostima
2023. urn:nbn:hr:227:918817
Matak, Lucija
University of Split
University Department for Forensic Sciences

Cite this document

Matak, L. (2023). Primjena direktne STR amplifikacije u forenzičnim znanostima (Master's thesis). Split: University of Split, University Department for Forensic Sciences. Retrieved from https://urn.nsk.hr/urn:nbn:hr:227:918817

Matak, Lucija. "Primjena direktne STR amplifikacije u forenzičnim znanostima." Master's thesis, University of Split, University Department for Forensic Sciences, 2023. https://urn.nsk.hr/urn:nbn:hr:227:918817

Matak, Lucija. "Primjena direktne STR amplifikacije u forenzičnim znanostima." Master's thesis, University of Split, University Department for Forensic Sciences, 2023. https://urn.nsk.hr/urn:nbn:hr:227:918817

Matak, L. (2023). 'Primjena direktne STR amplifikacije u forenzičnim znanostima', Master's thesis, University of Split, University Department for Forensic Sciences, accessed 21 April 2024, https://urn.nsk.hr/urn:nbn:hr:227:918817

Matak L. Primjena direktne STR amplifikacije u forenzičnim znanostima [Master's thesis]. Split: University of Split, University Department for Forensic Sciences; 2023 [cited 2024 April 21] Available at: https://urn.nsk.hr/urn:nbn:hr:227:918817

L. Matak, "Primjena direktne STR amplifikacije u forenzičnim znanostima", Master's thesis, University of Split, University Department for Forensic Sciences, Split, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:227:918817

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Metadata
TitlePrimjena direktne STR amplifikacije u forenzičnim znanostima
Title (english)Direct STR amplification in forensic science
AuthorLucija Matak
MentorDamir Marjanović (mentor)
MentorJosip Crnjac (komentor)
Committee memberSnježana Štambuk (predsjednik povjerenstva)
Committee memberNevena Aljinović (član povjerenstva)
Committee memberDamir Marjanović (član povjerenstva)
GranterUniversity of Split
University Department for Forensic Sciences
Split
Defense date and country2023-09-13, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Dobivanje potpunog DNK profila osobe iz tragova obično uključuje procese ekstrakcije DNK, njene kvantifikacije te amplificiranje STR lokusa. Međutim, iako u drugom koraku, ekstrakcije DNK dolazi i do njene purifikacije i uklanjanja inhibitora koji ometaju PCR reakciju, u tom procesu može doći do gubitka DNK. Ponekad se može izgubiti i do 87% DNK što smanjuje potencijal za generiranje profila za uzorke u kojima je početna količina DNK jako mala. Kako bi se smanjila mogućnost gubitka DNK za PCR reakciju provedena su istraživanja koja istražuju postupke u kojima se potpuno isključuje zaseban korak ekstrakcije DNK iz tragova. Naime, model direktnog PCR-a uključuje izravnu amplifikaciju STR lokusa iz uzoraka. Do sada ta je metoda ispitana na uzorcima forenzične važnosti kao što su krv, dlake, nokti, vlakna tkanina te otisci prstiju. Na temelju tih eksperimentalnih radova navedene su prednosti i mane ove metode i aspekti metode koje je potrebno dodatno usavršiti.
Abstract (english)
Obtaining a complete DNA profile of an individual from traces typically involves processes such as DNA extraction, quantification and amplification of STR loci. However, even if in the second step, DNA extraction is followed by purification and the removal of inhibitors that can interfere with the PCR reaction, this process can lead to DNA loss. In some cases, up to 87% of the DNA can be lost, reducing the potential for generating profiles for samples with very low initial DNA quantities. To reduce the risk of DNA loss in the PCR reaction, research has been conducted to explore methods that completely eliminate the separate DNA extraction step from traces. Specifically, the direct PCR model involves the direct amplification of STR loci from samples. So far, this method has been tested on forensically relevant samples such as blood, hair, nails, fabric fibers, and fingerprints. Based on these experimental works, the advantages and disadvantages of this method are outlined, along with aspects that require further refinement.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:227:918817
Study programmeTitle: Forensic Sciences
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra forenzike (magistar/magistra forenzike)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2024-02-05 18:01:23